It depends on how big your usual FCS file is, how many you have open, and how many gates and stats you apply.
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The speed at which this is done depends a lot on the software design decisions of the programmer. There can be a lot of computation that is involved when loading FCS files and calculating gates and statistics. Specifically, the images you see on visuals will be a display of this file with SSC-A on the Y-Axis and FSC-A on the X-axis. We use these FCS test files to evaluate the analysis software. The images displayed will be the default view that each program gives for a dot plot and histogram. So, how nice does the data look? We will open up a test file in each program and give you screenshots of dot plots and histograms. One of the major purposes of flow cytometry analysis software is to visualize data. We see how each of the programs stack up. Finally, there are some very specific features that most people don't really use at all. Then there are some nice to have features that are helpful in a lot of situations. There are a lot of features that are pretty standard that all flow cytometry analysis software should be able to perform. What does it take to get a dot plot to show up on the screen? How easy is it to draw gates or change the axis? What resources do the authors provide to help you through the process of getting started with their program? Features
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What dependencies does it have that the user needs to set up? How much personal information does the user need to provide? Is the software web-based or does it require you to download executables? Ease of Use We will take a look at what it takes to get started using the software. We will give a brief, high-level description of the important software design decisions that were made by the software programmers and discuss the trades offs of those decisions in general terms. We were programmers long before we did flow cytometry. We will look at and comment on the following aspects of each piece of software: Software Design However, it is missing a few newer software packages. It also contains a lot of software that is either really old and meant to be used with old file formats and data, or software that is very specialized to do a specific task. It has a lot of great software on it that we have found really useful.
The page gives a good overview of many different types of resources. Paul Robinson's software catalog over at Purdue University Cytometry Laboratories.
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Even though this paper is several years old at this point, most of the software they reviewed has not received any updates since they published this, so most of their points still apply.Īnother often-cited resource for free flow cytometry software information is J. (2014) took a look a 4 different free software applications in a paper you can find here. There are a couple of other places to find reviews of free flow cytometry software. We are here to go through the biggest and most complete free flow cytometry software available and discuss their pros and cons. There have been several projects that have sought out to create a free software analysis package for FCS files. Given the importance of flow cytometry to so many different scientific fields in today's world, analysis software that is usable by all should exist.
This is acceptable for large labs and big companies, but smaller groups who are more limited on resources may not be able to use them. Even with a paid license, there are usually limits placed on the number or location of computers that the software can be used on. Most of the time the price is so big that they don't even put the quote on their website. Unfortunately, they cost hundreds to thousands of dollars. There are many great commercially available software applications that allow not only for data visualization but also advanced statistical analysis of the data. Easy free ways to view flow cytometry FCS files are not.
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